Concentration of interleukin-1beta and neutrophil elastase activity in gingival crevicular fluid during experimental gingivitis.
Identifieur interne : 000519 ( Main/Exploration ); précédent : 000518; suivant : 000520Concentration of interleukin-1beta and neutrophil elastase activity in gingival crevicular fluid during experimental gingivitis.
Auteurs : J R Gonzáles [Allemagne] ; J M Herrmann ; R H Boedeker ; P I Francz ; H. Biesalski ; J. MeyleSource :
- Journal of clinical periodontology [ 0303-6979 ] ; 2001.
Descripteurs français
- KwdFr :
- Adulte (MeSH), Exsudat gingival (composition chimique), Exsudat gingival (enzymologie), Exsudat gingival (immunologie), Gingivite (enzymologie), Gingivite (immunologie), Gingivite (métabolisme), Humains (MeSH), Indice de plaque dentaire (MeSH), Indice parodontal (MeSH), Interleukine-1 (analyse), Leukocyte elastase (analyse), Mâle (MeSH), Papier (MeSH), Statistique non paramétrique (MeSH), Statistiques comme sujet (MeSH), Test ELISA (MeSH), Études de suivi (MeSH).
- MESH :
- analyse : Interleukine-1, Leukocyte elastase.
- composition chimique : Exsudat gingival.
- enzymologie : Exsudat gingival, Gingivite.
- immunologie : Exsudat gingival, Gingivite.
- métabolisme : Gingivite.
- Adulte, Humains, Indice de plaque dentaire, Indice parodontal, Mâle, Papier, Statistique non paramétrique, Statistiques comme sujet, Test ELISA, Études de suivi.
English descriptors
- KwdEn :
- Adult (MeSH), Dental Plaque Index (MeSH), Enzyme-Linked Immunosorbent Assay (MeSH), Follow-Up Studies (MeSH), Gingival Crevicular Fluid (chemistry), Gingival Crevicular Fluid (enzymology), Gingival Crevicular Fluid (immunology), Gingivitis (enzymology), Gingivitis (immunology), Gingivitis (metabolism), Humans (MeSH), Interleukin-1 (analysis), Leukocyte Elastase (analysis), Male (MeSH), Paper (MeSH), Periodontal Index (MeSH), Statistics as Topic (MeSH), Statistics, Nonparametric (MeSH).
- MESH :
- chemical , analysis : Interleukin-1, Leukocyte Elastase.
- chemistry : Gingival Crevicular Fluid.
- enzymology : Gingival Crevicular Fluid, Gingivitis.
- immunology : Gingival Crevicular Fluid, Gingivitis.
- metabolism : Gingivitis.
- Adult, Dental Plaque Index, Enzyme-Linked Immunosorbent Assay, Follow-Up Studies, Humans, Male, Paper, Periodontal Index, Statistics as Topic, Statistics, Nonparametric.
Abstract
BACKGROUND/AIM
The aim of the present study was to measure interleukin-1beta concentrations and neutrophil elastase activity in gingival crevicular fluid (GCF) during experimental gingivitis in humans.
MATERIAL AND METHODS
12 healthy young men participated. After prophylaxis, they performed optimal hygiene to reach plaque and gingivitis indices of or approaching zero. All oral hygiene measures were then ceased for a period of 18 days. The Quigley-Hein plaque index (PLI) and Saxer & Mühlemann papillary bleeding index (PBI) were assessed. GCF samples were taken from the mesiobuccal site of two contralateral teeth in the upper jaw by means of periopapers at baseline and on days 3, 7, 14 and 18. After measuring the gingival crevicular fluid volume (GCFV) with the Periotron 8000, the samples were analyzed in our laboratory for the detection of IL-1beta concentration by ELISA.
RESULTS
PLI and PBI showed a reduction prior to baseline reaching almost zero, both increasing from day 0 to day 18 (PLI=from 0.1 to 2.9, PBI=from 0 to 2.0). IL-1beta concentration increased from 229.25 ng/ml (day 0) to 526.13 ng/ml (day 18). Clinical data and IL-1beta concentrations were correlated with elastase activity (EA). No significant correlation could be demonstrated between the clinical parameters assessed and IL-1beta or EA (Spearman rank correlation coefficient). A correlation between GCFV and PBI from day 0 to day 18 could be demonstrated.
CONCLUSION
Overall, both IL-1beta and EA showed an increase from baseline throughout the whole study.
DOI: 10.1034/j.1600-051x.2001.028006544.x
PubMed: 11350521
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Concentration of interleukin-1beta and neutrophil elastase activity in gingival crevicular fluid during experimental gingivitis.</title><author><name sortKey="Gonzales, J R" sort="Gonzales, J R" uniqKey="Gonzales J" first="J R" last="Gonzáles">J R Gonzáles</name><affiliation wicri:level="1"><nlm:affiliation>Department of Periodontology, University of Giessen, Germany. jose.gonzales@dentist.med.uni-giessen.de</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Periodontology, University of Giessen</wicri:regionArea><wicri:noRegion>University of Giessen</wicri:noRegion><wicri:noRegion>University of Giessen</wicri:noRegion><wicri:noRegion>University of Giessen</wicri:noRegion></affiliation></author><author><name sortKey="Herrmann, J M" sort="Herrmann, J M" uniqKey="Herrmann J" first="J M" last="Herrmann">J M Herrmann</name></author><author><name sortKey="Boedeker, R H" sort="Boedeker, R H" uniqKey="Boedeker R" first="R H" last="Boedeker">R H Boedeker</name></author><author><name sortKey="Francz, P I" sort="Francz, P I" uniqKey="Francz P" first="P I" last="Francz">P I Francz</name></author><author><name sortKey="Biesalski, H" sort="Biesalski, H" uniqKey="Biesalski H" first="H" last="Biesalski">H. Biesalski</name></author><author><name sortKey="Meyle, J" sort="Meyle, J" uniqKey="Meyle J" first="J" last="Meyle">J. Meyle</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2001">2001</date><idno type="RBID">pubmed:11350521</idno><idno type="pmid">11350521</idno><idno type="doi">10.1034/j.1600-051x.2001.028006544.x</idno><idno type="wicri:Area/Main/Corpus">000521</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000521</idno><idno type="wicri:Area/Main/Curation">000521</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000521</idno><idno type="wicri:Area/Main/Exploration">000521</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Concentration of interleukin-1beta and neutrophil elastase activity in gingival crevicular fluid during experimental gingivitis.</title><author><name sortKey="Gonzales, J R" sort="Gonzales, J R" uniqKey="Gonzales J" first="J R" last="Gonzáles">J R Gonzáles</name><affiliation wicri:level="1"><nlm:affiliation>Department of Periodontology, University of Giessen, Germany. jose.gonzales@dentist.med.uni-giessen.de</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Periodontology, University of Giessen</wicri:regionArea><wicri:noRegion>University of Giessen</wicri:noRegion><wicri:noRegion>University of Giessen</wicri:noRegion><wicri:noRegion>University of Giessen</wicri:noRegion></affiliation></author><author><name sortKey="Herrmann, J M" sort="Herrmann, J M" uniqKey="Herrmann J" first="J M" last="Herrmann">J M Herrmann</name></author><author><name sortKey="Boedeker, R H" sort="Boedeker, R H" uniqKey="Boedeker R" first="R H" last="Boedeker">R H Boedeker</name></author><author><name sortKey="Francz, P I" sort="Francz, P I" uniqKey="Francz P" first="P I" last="Francz">P I Francz</name></author><author><name sortKey="Biesalski, H" sort="Biesalski, H" uniqKey="Biesalski H" first="H" last="Biesalski">H. Biesalski</name></author><author><name sortKey="Meyle, J" sort="Meyle, J" uniqKey="Meyle J" first="J" last="Meyle">J. Meyle</name></author></analytic><series><title level="j">Journal of clinical periodontology</title><idno type="ISSN">0303-6979</idno><imprint><date when="2001" type="published">2001</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult (MeSH)</term><term>Dental Plaque Index (MeSH)</term><term>Enzyme-Linked Immunosorbent Assay (MeSH)</term><term>Follow-Up Studies (MeSH)</term><term>Gingival Crevicular Fluid (chemistry)</term><term>Gingival Crevicular Fluid (enzymology)</term><term>Gingival Crevicular Fluid (immunology)</term><term>Gingivitis (enzymology)</term><term>Gingivitis (immunology)</term><term>Gingivitis (metabolism)</term><term>Humans (MeSH)</term><term>Interleukin-1 (analysis)</term><term>Leukocyte Elastase (analysis)</term><term>Male (MeSH)</term><term>Paper (MeSH)</term><term>Periodontal Index (MeSH)</term><term>Statistics as Topic (MeSH)</term><term>Statistics, Nonparametric (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adulte (MeSH)</term><term>Exsudat gingival (composition chimique)</term><term>Exsudat gingival (enzymologie)</term><term>Exsudat gingival (immunologie)</term><term>Gingivite (enzymologie)</term><term>Gingivite (immunologie)</term><term>Gingivite (métabolisme)</term><term>Humains (MeSH)</term><term>Indice de plaque dentaire (MeSH)</term><term>Indice parodontal (MeSH)</term><term>Interleukine-1 (analyse)</term><term>Leukocyte elastase (analyse)</term><term>Mâle (MeSH)</term><term>Papier (MeSH)</term><term>Statistique non paramétrique (MeSH)</term><term>Statistiques comme sujet (MeSH)</term><term>Test ELISA (MeSH)</term><term>Études de suivi (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Interleukin-1</term><term>Leukocyte Elastase</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Interleukine-1</term><term>Leukocyte elastase</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Gingival Crevicular Fluid</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Exsudat gingival</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Exsudat gingival</term><term>Gingivite</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Gingival Crevicular Fluid</term><term>Gingivitis</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Exsudat gingival</term><term>Gingivite</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Gingival Crevicular Fluid</term><term>Gingivitis</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Gingivitis</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Gingivite</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Dental Plaque Index</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Follow-Up Studies</term><term>Humans</term><term>Male</term><term>Paper</term><term>Periodontal Index</term><term>Statistics as Topic</term><term>Statistics, Nonparametric</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte</term><term>Humains</term><term>Indice de plaque dentaire</term><term>Indice parodontal</term><term>Mâle</term><term>Papier</term><term>Statistique non paramétrique</term><term>Statistiques comme sujet</term><term>Test ELISA</term><term>Études de suivi</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND/AIM</b></p><p>The aim of the present study was to measure interleukin-1beta concentrations and neutrophil elastase activity in gingival crevicular fluid (GCF) during experimental gingivitis in humans.</p></div><div type="abstract" xml:lang="en"><p><b>MATERIAL AND METHODS</b></p><p>12 healthy young men participated. After prophylaxis, they performed optimal hygiene to reach plaque and gingivitis indices of or approaching zero. All oral hygiene measures were then ceased for a period of 18 days. The Quigley-Hein plaque index (PLI) and Saxer & Mühlemann papillary bleeding index (PBI) were assessed. GCF samples were taken from the mesiobuccal site of two contralateral teeth in the upper jaw by means of periopapers at baseline and on days 3, 7, 14 and 18. After measuring the gingival crevicular fluid volume (GCFV) with the Periotron 8000, the samples were analyzed in our laboratory for the detection of IL-1beta concentration by ELISA.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>PLI and PBI showed a reduction prior to baseline reaching almost zero, both increasing from day 0 to day 18 (PLI=from 0.1 to 2.9, PBI=from 0 to 2.0). IL-1beta concentration increased from 229.25 ng/ml (day 0) to 526.13 ng/ml (day 18). Clinical data and IL-1beta concentrations were correlated with elastase activity (EA). No significant correlation could be demonstrated between the clinical parameters assessed and IL-1beta or EA (Spearman rank correlation coefficient). A correlation between GCFV and PBI from day 0 to day 18 could be demonstrated.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSION</b></p><p>Overall, both IL-1beta and EA showed an increase from baseline throughout the whole study.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11350521</PMID><DateCompleted><Year>2001</Year><Month>10</Month><Day>25</Day></DateCompleted><DateRevised><Year>2019</Year><Month>09</Month><Day>01</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0303-6979</ISSN><JournalIssue CitedMedium="Print"><Volume>28</Volume><Issue>6</Issue><PubDate><Year>2001</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Journal of clinical periodontology</Title><ISOAbbreviation>J Clin Periodontol</ISOAbbreviation></Journal><ArticleTitle>Concentration of interleukin-1beta and neutrophil elastase activity in gingival crevicular fluid during experimental gingivitis.</ArticleTitle><Pagination><MedlinePgn>544-9</MedlinePgn></Pagination><Abstract><AbstractText Label="BACKGROUND/AIM" NlmCategory="OBJECTIVE">The aim of the present study was to measure interleukin-1beta concentrations and neutrophil elastase activity in gingival crevicular fluid (GCF) during experimental gingivitis in humans.</AbstractText><AbstractText Label="MATERIAL AND METHODS" NlmCategory="METHODS">12 healthy young men participated. After prophylaxis, they performed optimal hygiene to reach plaque and gingivitis indices of or approaching zero. All oral hygiene measures were then ceased for a period of 18 days. The Quigley-Hein plaque index (PLI) and Saxer & Mühlemann papillary bleeding index (PBI) were assessed. GCF samples were taken from the mesiobuccal site of two contralateral teeth in the upper jaw by means of periopapers at baseline and on days 3, 7, 14 and 18. After measuring the gingival crevicular fluid volume (GCFV) with the Periotron 8000, the samples were analyzed in our laboratory for the detection of IL-1beta concentration by ELISA.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">PLI and PBI showed a reduction prior to baseline reaching almost zero, both increasing from day 0 to day 18 (PLI=from 0.1 to 2.9, PBI=from 0 to 2.0). IL-1beta concentration increased from 229.25 ng/ml (day 0) to 526.13 ng/ml (day 18). Clinical data and IL-1beta concentrations were correlated with elastase activity (EA). No significant correlation could be demonstrated between the clinical parameters assessed and IL-1beta or EA (Spearman rank correlation coefficient). A correlation between GCFV and PBI from day 0 to day 18 could be demonstrated.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">Overall, both IL-1beta and EA showed an increase from baseline throughout the whole study.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Gonzáles</LastName><ForeName>J R</ForeName><Initials>JR</Initials><AffiliationInfo><Affiliation>Department of Periodontology, University of Giessen, Germany. jose.gonzales@dentist.med.uni-giessen.de</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Herrmann</LastName><ForeName>J M</ForeName><Initials>JM</Initials></Author><Author ValidYN="Y"><LastName>Boedeker</LastName><ForeName>R H</ForeName><Initials>RH</Initials></Author><Author ValidYN="Y"><LastName>Francz</LastName><ForeName>P I</ForeName><Initials>PI</Initials></Author><Author ValidYN="Y"><LastName>Biesalski</LastName><ForeName>H</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Meyle</LastName><ForeName>J</ForeName><Initials>J</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Clin Periodontol</MedlineTA><NlmUniqueID>0425123</NlmUniqueID><ISSNLinking>0303-6979</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007375">Interleukin-1</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.4.21.37</RegistryNumber><NameOfSubstance UI="D019272">Leukocyte Elastase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>D</CitationSubset><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003774" MajorTopicYN="N">Dental Plaque Index</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005500" MajorTopicYN="N">Follow-Up Studies</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005883" MajorTopicYN="N">Gingival Crevicular Fluid</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005891" MajorTopicYN="N">Gingivitis</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007375" MajorTopicYN="N">Interleukin-1</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019272" MajorTopicYN="N">Leukocyte Elastase</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010209" MajorTopicYN="N">Paper</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010512" MajorTopicYN="N">Periodontal Index</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013223" MajorTopicYN="N">Statistics as Topic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018709" MajorTopicYN="N">Statistics, Nonparametric</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2001</Year><Month>5</Month><Day>15</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2001</Year><Month>10</Month><Day>26</Day><Hour>10</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2001</Year><Month>5</Month><Day>15</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">11350521</ArticleId><ArticleId IdType="pii">cpe280606</ArticleId><ArticleId IdType="doi">10.1034/j.1600-051x.2001.028006544.x</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country></list><tree><noCountry><name sortKey="Biesalski, H" sort="Biesalski, H" uniqKey="Biesalski H" first="H" last="Biesalski">H. Biesalski</name><name sortKey="Boedeker, R H" sort="Boedeker, R H" uniqKey="Boedeker R" first="R H" last="Boedeker">R H Boedeker</name><name sortKey="Francz, P I" sort="Francz, P I" uniqKey="Francz P" first="P I" last="Francz">P I Francz</name><name sortKey="Herrmann, J M" sort="Herrmann, J M" uniqKey="Herrmann J" first="J M" last="Herrmann">J M Herrmann</name><name sortKey="Meyle, J" sort="Meyle, J" uniqKey="Meyle J" first="J" last="Meyle">J. Meyle</name></noCountry><country name="Allemagne"><noRegion><name sortKey="Gonzales, J R" sort="Gonzales, J R" uniqKey="Gonzales J" first="J R" last="Gonzáles">J R Gonzáles</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SuicidDentistV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000519 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000519 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SuicidDentistV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:11350521
|texte= Concentration of interleukin-1beta and neutrophil elastase activity in gingival crevicular fluid during experimental gingivitis.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:11350521" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SuicidDentistV1
| This area was generated with Dilib version V0.6.39. |  |